leptin  (Technology Development Co)

Journal: Cardiovascular diabetology. Endocrinology reports

Article Title: Diabetes-induced right ventricular remodeling precedes left ventricular remodeling and occurs via a distinct fatty acid uptake pathway

doi: 10.1186/s40842-026-00287-3

Figure Lengend Snippet: Quantification of fatty acid uptake in the RV. ( A ) BODIPY staining of FA in the RV showed no difference between db/db and WT mice. Representative images of BODIPY stained RV sections. ( B ) Quantification of the cardiomyocyte fatty acid transporter, CD36 was lower in the db/db RV compared to the WT. ( C ) CD36 quantification by western blot showed no difference in the db/db RV. Representative western blots. ( D ) Membrane localization of CD36 showed downregulation in db/db RV. ( E ) Gene expression of FATP6 was upregulated in the db/db RV. ( F ) Protein expression by western blot showed higher FATP6 expression in the db/db RV. Representative western blots of FATP6 and caveolin. ( G ) Protein localization of FATP6 at the membrane was higher in the db/db RV compared to WT. ( H ) Quantification of enzymes involved in fatty acid oxidation. Acyl CoA dehydrogenases long chain and medium chain (ACADl, ACADm, ACS1) and acyl CoA synthetases 1 (ACS1). ACADl was lower in the db/db RV, mACADm and ACS1 were unchanged in the db/db RV. * p < 0.05 by Student’s t-test. n = 4/group. Scale bar: 50 µm

Article Snippet: Male and female 6–8 week old leptin receptor mutant (db/db) that are homozygous for a spontaneous mutation in the leptin receptor [ ] and heterozygote lean littermates (wild type, WT) were ordered from Jackson Laboratories (#00697).

Techniques: Staining, Western Blot, Membrane, Gene Expression, Expressing

Journal: Cardiovascular diabetology. Endocrinology reports

Article Title: Diabetes-induced right ventricular remodeling precedes left ventricular remodeling and occurs via a distinct fatty acid uptake pathway

doi: 10.1186/s40842-026-00287-3

Figure Lengend Snippet: RV remodeling in 8–10 week old db/db mice. ( A ) RV weight normalized to tibia length (TL) was higher compared to WT. ( B ) RV anterior wall (RVAW) thickness was higher in diastole in the db/db mice compared to the WT and trended towards significance during systole. ( C ) RV myocyte cross-sectional area (CSA) remained the same in the db/db RV, n = 20. Representative images of lectin staining. ( D ) Quantification of fibrosis by picrosirius red staining showed higher collagen in the db/db RV compared to WT. Representative images of PSR staining. ( F ) Quantification of lipid accumulation by oil red O staining showed higher lipid accumulation in the db/db RV compared to the WT. Representative images of oil red O staining. * p < 0.05 by Student’s t-test. For oil red O and picrosirius red staining, n = 3 with at least three views quantified per image. Scale bar: 50 µm

Article Snippet: Male and female 6–8 week old leptin receptor mutant (db/db) that are homozygous for a spontaneous mutation in the leptin receptor [ ] and heterozygote lean littermates (wild type, WT) were ordered from Jackson Laboratories (#00697).

Techniques: Staining

Journal: Cardiovascular diabetology. Endocrinology reports

Article Title: Diabetes-induced right ventricular remodeling precedes left ventricular remodeling and occurs via a distinct fatty acid uptake pathway

doi: 10.1186/s40842-026-00287-3

Figure Lengend Snippet: Functional assessment of RV remodeling in T2D mice by echocardiography. ( A ) Representative images from M-mode echocardiography comparing WT and db/db RV. ( B ) RV FAC was lower in the db/db RV alongside ( C ) lower PA-VTI, suggestive of impaired systolic function. ( D ) RV E/e’ was higher in the db/db RV. ( E ) Representative images from M-mode echocardiography comparing Con and T2D RV. ( F ) Lower RV FAC and ( G ) TAPSE in T2D compared to the Con were indicative of impaired RV systolic function. * p < 0.05 by Student’s t-test. n = 5–11 per group

Article Snippet: Male and female 6–8 week old leptin receptor mutant (db/db) that are homozygous for a spontaneous mutation in the leptin receptor [ ] and heterozygote lean littermates (wild type, WT) were ordered from Jackson Laboratories (#00697).

Techniques: Functional Assay